Monthly Archives: April 2011

The skin and LSJL

Since the skin is under contact via LSJL it’s important to note whether LSJL will do any damage.  The goal pressure of LSJL is around 10 MPa.

The Rapid Inactivation of Porcine Skin by Applying High Hydrostatic Pressure without Damaging the Extracellular Matrix

“We previously reported that high hydrostatic pressure (HHP) of 200 MPa for 10 minutes could induce cell killing. In this study, we explored whether HHP at 200 MPa or HHP at lower pressure, in combination with hyposmotic distilled water (DW), could inactivate the skin, as well as cultured cells. We investigated the inactivation of porcine skin samples 4 mm in diameter. They were immersed in either a normal saline solution (NSS) or DW, and then were pressurized at 100 and 200 MPa for 5, 10, 30, or 60 min. Next, we explored the inactivation of specimens punched out from the pressurized skin 10 × 2 cm in size. The viability was evaluated using a WST-8 assay and an outgrowth culture. The histology of specimens was analyzed histologically. The mitochondrial activity was inactivated after the pressurization at 200 MPa in both experiments, and no outgrowth was observed after the pressurization at 200 MPa. The arrangement and proportion of the dermal collagen fibers or the elastin fibers were not adversely affected after the pressurization at 200 MPa for up to 60 minutes. This study showed that a HHP at 200 MPa for 10 min could inactivate the skin without damaging the dermal matrix.”

So basically the 10 MPa goal is well under the 200 MPa that “damages” the skin.  And really it just inactivates mitochondrial activity.

” high hydrostatic pressure (HHP) of more than 600 MPa for 10 minutes could destroy cell membranes uniformly and in a short treatment time (within one hour, including the increasing and decreasing process of pressure), regardless of the thickness or hardness of the tissue ”

Here’s the fibrostic cells in response to 0 to 200 HP:   No more chondrogenic looking than it appeared before unfortunately.