This was an idea that I have been thinking about ever since I found and reviewed the study which showed that BMP-7 (aka OP-1) injections into a mice and sheep’s vertebrate lead to the regain of lost height and health of degenerating disks.
My idea is to create a type of padding or guase which you would have BMP-7 and BMP-6 with TGF-Beta3 embedded in the padding. Dexamethasone might also help. Certain BMPs and growth factors have been shown to both lead to chondrogenesis and osteogenesis. BMP-6 is really good at osteogenesis. We are looking for those. The padding has these notches and bumps, with the bumps alligned with the location of the intervertebral disks. The padding is pressed agains tthe back skin, with small needles that can inject the growth factors right next to the disks. Remember that the disks have an outer concentric layer (outer annulus fibrosus) and the inner circular layer (nucleus pulposus (NP). The outer layer is what the growth factors have to both get around and into. There is still some cartilage in the vertebrate even after we have reached physical maturity.
The padding can be just even 1 cm thick and can be refilled like a syringe which the nurse replaces when she is collecting a lot of blood from you. The BMP will theoretically be soaked into the vertebral disks leading to first chondrogenesis, increased nucleus pulposus function (and maybe even size), and finally ossification leading to increased thickness in the disks causing torso and height lengthening. The sequence of body physiology can be controlled by manipulating the injected device to switch from one type of growth factor into another. The pads can be wrapped around the body with either velcro or an elastic strap.
The second part of the idea was to use PEMF technology on the sides of the disks and give short duration, low intensity sine wave electrically capacitive signals to stimulate the collagenous material… At this point I would like to test the PEMF idea on the collagenous fibers of the annulus fibrosus so see if they would get any larger.
Overall, this idea is rather simple to try out even at home. The hard part may be getting BMP-7 and the TGF-Beta 3 from a non-natural source.
From PubMed Study 1 HERE
Spine J. 2008 May-Jun;8(3):466-74. Epub 2007 Jun 26.
Bone morphogenetic protein-7 protects human intervertebral disc cells in vitro from apoptosis.
Orthopaedic Research Institute, The University of New South Wales, St George Hospital Campus, level 2, 4-10 South Street, Kogarah, NSW 2217, Australia.
Disc degeneration includes dysfunction and loss of disc cells leading to a decrease in extracellular matrix (ECM) components. Apoptosis has been identified in degenerated discs. Bone morphogenetic protein-7 (BMP-7) has been reported to stimulate ECM synthesis in the intervertebral disc (IVD), but its effect on disc cell viability is unknown.
To investigate whether BMP-7 can protect disc cells from programmed cell death while enhancing ECM production.
An in vitro study to examine the effect of BMP-7 on apoptosis of IVD cells.
Human nucleus pulposus (NP) cells were cultured in monolayer, and human recombinant pure BMP-7 (rhBMP-7) was added to the medium when the cells were in the second passage. Thereafter, apoptosis was induced by either tumor necrosis factor-alpha (TNF-alpha) or hydrogen peroxide (H(2)O(2)). Cellular apoptosis was evaluated by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay and caspase-3 activity. ECM synthesis was assessed by immunofluorescence for collagen-2 and aggrecan. To study the possibility of bone induction by rhBMP-7 in disc cells, alkaline phosphatase activity and Alizarin red-S staining were evaluated.
Apoptosis was induced by both TNF-alpha and H(2)O(2). Addition of rhBMP-7 resulted in inhibition of the apoptotic effects caused by both inducers. Further, BMP-7 decreased caspase-3 activity. In the presence of BMP-7, ECM production was maintained by the cells despite being in an apoptotic environment. No osteoblastic induction of the disc cells was seen.
BMP-7 was demonstrated to prevent apoptosis of human disc cells in vitro. One of the antiapoptotic effects of BMP-7 on NP cells might be a result of its inactivation of caspase-3. Collagen production was maintained by addition of rhBMP-7 in an apoptotic environment.
- PMID: 18082466 [PubMed – indexed for MEDLINE]
From PubMed study 2 HERE
- Tissue Eng Part B Rev. 2010 Aug;16(4):435-44.
Comparative review of growth factors for induction of three-dimensional in vitro chondrogenesis in human mesenchymal stem cells isolated from bone marrow and adipose tissue.
Joint Department of Biomedical Engineering at UNC-Chapel Hill and North Carolina State University, Raleigh, North Carolina 27695, USA.
The ability of bone-marrow-derived mesenchymal stem cells (MSCs) and adipose-derived stem cells (ASCs) to undergo chondrogenic differentiation has been studied extensively, and it has been suggested that the chondrogenic potential of these stem cells differ from each other. Here, we provide a comprehensive review and analysis of the various growth factor induction agents for MSC and ASC three-dimensional in vitro chondrogenic differentiation. In general, the most common growth factors for chondrogenic induction come from the transforming growth factor beta (TGFbeta) superfamily. To date, the most promising growth factors for chondrogenesis appear to be TGFbeta-3 and bone morphogenetic protein (BMP)-6. A thorough review of the literature indicates that human MSCs (hMSCs) appear to exhibit the highest chondrogenic potential in three-dimensional culture in the medium containing both dexamethasone and TGFbeta-3. Some reports indicate that the addition of BMP-6 to TFGbeta-3 and dexamethasone further increases hMSC chondrogenesis, but these results are still not consistently supported. Induction of human ASC (hASC) chondrogenesis appears most successful when dexamethasone, TGFbeta-3, and BMP-6 are used in combination. However, to date, current formulations do not always result in stable differentiation to the chondrocytic lineage by hMSCs and hASCs. Continued research must be performed to examine the expression cascades of the TFGbeta superfamily to further determine the effects of each growth factor alone and in combination on these stem cell lines.
- PMID: 20196646 [PubMed – indexed for MEDLINE]