Using Dexamethasone And TGF-Beta1 To Turn Bone Marrow Derived Mesenchymal Progenitor Cells Into Chondrocytes

Me: What this study has shown is another method to turn bone marrow derived mesenchymal progenitor cells. Remember, this is not for adiposed derived adult stem cells, but bone marrow drived mesenchymal progenitor cells. There is a difference, and if I do try to use growth factor combinations to induce height increase, we have to know how the process should happen. This study involved trying out the two growth factors dexamethasone and TGF-Beta1. With the deaxamethasone we are seeing some developed of chondrogenesis as well as collagen type II formation. With time, the type II turns into type X from the obvious differentiation of the chondrocytes into hypertrophic in nature. Besides just using the dexamethasone some TGF-Beta 1 was also added which resulted in call cell culture samples undergoing chondrogenesis. There is an increase in the alkaline phosphatase activity. there is evidence for collagen type II a and type II b and type X. interestingly, type I collagen mRNA is no longer detected.

What this implies: In three of the most recent posts I have looked at the effectiveness of using TGF-1, BMP-3, Dexamethasone, and Chitosan. You can encapsulate the BMP-6, TGF-Beta 1 with Chitosan, and the TGF-Beta 1 with dexamethasone. We are finding like from other studies that the TGF-1 (or TGF-2) works very well with other types of chondroinductive material. I would suggest than first just sending in the TGF-1 with chitosan, then add in the deaxamethasone, and then the BMP-6. It would be interesting to see what would be the result if we tried to multiply the chondrogenetic qualities of all of these growth factors together. With the dexamethasone we should be able to add this into the epiphysis and not just the bone marrow.

From PubMed study link HERE

Exp Cell Res. 1998 Jan 10;238(1):265-72.

In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells.

Johnstone B, Hering TM, Caplan AI, Goldberg VM, Yoo JU.

Source

Skeletal Research Center, Case Western Reserve University, Cleveland, Ohio 44106, USA. bxj9@po.cwru.edu

Abstract

A culture system that facilitates the chondrogenic differentiation of rabbit bone marrow-derived mesenchymal progenitor cells has been developed. Cells obtained in bone marrow aspirates were first isolated by monolayer culture and then transferred into tubes and allowed to form three-dimensional aggregates in a chemically defined medium. The inclusion of 10(-7) M dexamethasone in the medium induced chondrogenic differentiation of cells within the aggregate as evidenced by the appearance of toluidine blue metachromasia and the immunohistochemical detection of type II collagen as early as 7 days after beginning three-dimensional culture. After 21 days, the matrix of the entire aggregate contained type II collagen. By 14 days of culture, there was also evidence for type X collagen present in the matrix and the cells morphologically resembled hypertrophic chondrocytes. However, chondrogenic differentiation was achieved in only approximately 25% of the marrow cell preparations used. In contrast, with the addition of transforming growth factor-beta 1 (TGF-beta 1), chondrogenesis was induced in all marrow cell preparations, with or without the presence of 10(-7) M dexamethasone. The induction of chondrogenesis was accompanied by an increase in the alkaline phosphatase activity of the aggregated cells. The results of RT-PCR experiments indicated that both type IIA and IIB collagen mRNAs were detected by 7 days postaggregation as was mRNA for type X collagen. Conversely, the expression of the type I collagen mRNA was detected in the preaggregate cells but was no longer detectable at 7 days after aggregation. These results provide histological, immunohistochemical, and molecular evidence for the in vitro chondrogenic differentiation of adult mammalian progenitor cells derived from bone marrow.

PMID: 9457080    [PubMed – indexed for MEDLINE]

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