How Svetlana Zagirovna Sharifullina Developed A Vascularized Fully Functioning Growth Plate
Maybe a month ago I had written the post “More Information On Alexander Teplyashin and His Moscow Based Clinics” and said that Teplyashin is not the real mastermind behind the whole stem cell method of bone lengthening. He is a cosmetic plastic surgeon. His Ph. D was on plastic surgery and his specialty is towards minimally invasive approaches to help women have breast implants.
I had said that it is his assistant or colleague who is doing the real research on that. That person is Sharifullina Svetlana Zagirovna. If you type that name into Google, you will find nothing on this person, or very little. That is because you have to use the Russian based search engines and search for her russian name.
Here is what you need to look for in Google or Yandex.
Мультипотентные мезенхимные стромальные клетки жировой ткани и использование их в создании трехмерных трансплантатов хрящевой ткани
It translates into…
Multipotent mesenchymal stromal cells of adipose tissue and their use in the creation of three-dimensional cartilage grafts
The Ph. D Candidate’s real name: Шарифуллина, Светлана Загировна. This is what many people don’t realize. When you see Teplyashin’s name on the research papers, you also look at the other people’s names. There is almost always two other researcher’s names that is with him. They are…
- Korzhikova SV
- Sharifullina SZ
You can see that Sharifullina SZ is this person, who had done through Ph. D thesis on cartilage generation using TGF-beta and stem cells. Like any good leader, they surround themselves with people who are much smarter than themselves, at least in their respective areas of study.
I quote the following from the Ph.D translated by Google Chrome….
2.1.13. A method for obtaining three-dimensional cartilage grafts
Three-dimensional cartilage graft obtained by the method recommended by the manufacturer Biomatrix – BD Biosciences (Germany). The three-dimensional matrix based on polylactic acid (OPLA) or collagen (Collagen) was placed in a 15 ml tube, 150 ul of the cell suspension at a concentration of 5 x 104/ml was applied to the matrix and incubated with orbital shaking at +37 ° C for 2 hours. Then added differential tsirovochnuyu environment and placed in culture for a C02 incubator. Composition differentiating medium: DMEM-HG, 1% Albumin (HSA), 0,1 nM dexamethasone, 0.2 mM ascorbate-2-phosphate, 1 x ITS, 10 ng / ml TGF-J31, 1% antibiotic. Change carried out through the medium every 3 days for 4 weeks.
2.1.14. Preclinical trials – transplantation TTHT based ITCs adipose tissue in the body immunodeficient mice
Preclinical trials conducted in experimental animals, provided by the Russian State University Cancer Research Center. NI RAMS in a joint research agreement. Three-dimensional cartilage tissue grafts prepared in vitro, ectopically sutured back to immunodeficient mice (Balb / C nude) for a period of 8 weeks to assess toxicity and tumorigenicity. After completion of the experiment the animals were euthanized and the grafts removed. In the experiment guided by the requirements of the World Society for the Protection of Animals (WSPA) and the European Convention for the protection of experimental animals.
If you searched further, you’d find her Ph.D is used as a citation on Teplyashin’s personal websites, both StemCelRussia.com and BeautyPlaza.ru
The Ph.D was defended back in 2007 and it wasn’t until 2011 when another proposal for a project was made entitled “In vitro obtainment of bone marrow multipotent mesenchymal stromal cell three-dimensional bone tissue grafts and their application in bone tissue injury.”
This almost agrees exactly with what happened on the Make Me Taller Forums when the news of Teplyashin’s claims first came out maybe 3-4 years ago. That thread on MMT was very much talked about and people did get excited, but over the last few years the MMT crowd seems to have lost interest. It is a shame because based on the time line, I think the Teplyashin research team is actually quite on track in terms of research.
I know from experience that biomedical technology that can finally be taken out of the lab and used in treatments take decades to come about. The rate of the research is actually very, VERY fast. it has been only maybe 3-4 years, which is nothing. I said in the research post that when it comes to biomedical technology development, even hundreds of millions of dollars spent on research might eventually yield nothing in return.
(source: http://www.stemcellrussia.com/en_research_papers)
I also noticed another article Teplyashin’s team wrote up at the bottom of the webpage which caught my eye.
Teplyashin A.S. HUVEC for Prevascularization of Bone Tissue-Engineered Graft / Kulneva E.I., Korjikova S.V., Teplyashin A.S. // J Biotechnol. – 2010. – V.150. – S1. – P.96-97
It seems that maybe Teplyashin’s team figured out how to solve the vascularization problem that so many surgeons have had in doing epiphyseal growth plate transplantation. The big problem for over a century in getting growth plate transplants to work after implantation has always been that there is a vascularization problem. The scaffold with stem cells embedded inside could not get the blood vessels to wrap and go into the scaffolds and fuse together. It seems that they might have a way to figure that out.
If Zagirovna has been successful in getting the 3-D Cartilage Grafts to be grown, and they got the vascularization problem figure out, then what Teplyashin had been claiming is absolutely true.
